Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PAA1 Polyclonal antibody

Uniprot No.

Q9SZC9

Target Names

PAA1

Alternative Names

PAA1 antibody; GIR1 antibody; HMA6 antibody; At4g33520 antibody; F17M5.280 antibody; T16L1.10Copper-transporting ATPase PAA1 antibody; chloroplastic antibody; EC 7.2.2.8 antibody; Protein HEAVY METAL ATPASE 6 antibody; Protein glucose insensitive root 1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana PAA1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Mediates copper transfer across the plastid envelope. Required for the delivery of copper into the plastid stroma, which is essential for the function of copper proteins. Seems to be selective for monovalent copper Cu(+) transport. Plays also a role in glucose signaling-mediated cell proliferation of root meristem in non-green tissues.

Gene References into Functions

The crystal structures of the nucleotide binding domain of HMA6 and HMA8 from Arabidopsis thaliana are reported at 1.5A and 1.75A resolution, respectively, providing the first structural information on plants Cu+-ATPases. PMID: 27802305
Combining functional and structural data, we highlight the importance of the cysteine and the first histidine of the CX3HX2H motif in the process of copper release from HMA6 and HMA8 and propose a copper pathway through the membrane domain of these transporters. PMID: 27493208
HMA6 enzymatic properties and affinity for Cu PMID: 26182363
Plastid chaperone 1 (PCH1), which is produced as an alternative-splicing event of the PAA1 pre-mRNA, delivers Cu+ with specificity to PAA1. PMID: 25468978
HMA1 and PAA1 behave as distinct pathways for copper import and targeting to the chloroplast. PMID: 24510941
the plastidial Cu transporter PAA1, which is essential for plastid function and/or activity, plays an important role in bidirectional communication between the plastid and the nucleus in high glucose. PMID: 22582133
the first enzymatic characterization of a P(IB-1)-type plant ATPase and clearly identify PAA1 as a high affinity Cu(I) transporter of the chloroplast envelope. PMID: 21878617

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Subcellular Location

Plastid, chloroplast membrane; Multi-pass membrane protein.

Protein Families

Cation transport ATPase (P-type) (TC 3.A.3) family, Type IB subfamily

Tissue Specificity

Expressed in the shoots and roots.

Database Links

KEGG: ath:AT4G33520

STRING: 3702.AT4G33520.2

UniGene: At.48932

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PAA1 Antibody

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