HMG2 Antibody

Code CSB-PA320139XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) HMG2 Polyclonal antibody
Uniprot No.
Target Names
HMG2
Alternative Names
HMG2 antibody; YLR450W antibody; L9324.2 antibody; 3-hydroxy-3-methylglutaryl-coenzyme A reductase 2 antibody; HMG-CoA reductase 2 antibody; EC 1.1.1.34 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) HMG2 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
One of 2 isozymes that catalyze the conversion of HMG-CoA to mevalonate. It is the rate-limiting enzyme of the sterol biosynthesis pathway. Involved in ergosterol biosynthesis.
Gene References into Functions
  1. Nsg1-mediated stabilization of Hmg2 and the Nsg1-Hmg2 interaction required the early sterol lanosterol. PMID: 23306196
  2. The sterol-sensing domain (SSD) directly mediates signal-regulated endoplasmic reticulum-associated degradation (ERAD) of 3-hydroxy-3-methylglutaryl (HMG)-CoA reductase isozyme Hmg2. PMID: 21628456
  3. In the K6R substituted Hmg2 mutant, the squalene content was approximately 20-fold higher than in the wild-type EGY48 parental strain and ERG6 deletion did not further enhance squalene accumulation. PMID: 20550581
  4. results show that farnesol causes Hmg2p to undergo a change to a less folded structure PMID: 15635451
  5. although related, Hmg2p- and Hmg1p-induced ER remodeling are mechanistically distinct PMID: 18667535
  6. Hrd1p was rate-limiting for Hmg2p-GFP retrotranslocation, which required ATP, the AAA-ATPase Cdc48p, and its receptor Ubx2p. PMID: 19324879
  7. GGPP is the FPP-derived regulator of Hmg2p ubiquitination. PMID: 19776008

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Subcellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein. Nucleus envelope.
Protein Families
HMG-CoA reductase family
Database Links

KEGG: sce:YLR450W

STRING: 4932.YLR450W

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