Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) HMT1 Polyclonal antibody

Uniprot No.

P38074

Target Names

HMT1

Alternative Names

HMT1 antibody; ODP1 antibody; RMT1 antibody; YBR034C antibody; YBR0320 antibody; Protein arginine N-methyltransferase 1 antibody; EC 2.1.1.- antibody; Major type I protein arginine N-methyltransferase antibody; Type I PRMT antibody; hnRNP arginine N-methyltransferase antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) HMT1 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

S-adenosyl-L-methionine-dependent protein-arginine N-methyltransferase that catalyzes both the mono- and asymmetric (type I) dimethylation of the guanidino nitrogens of arginine residues in a variety of RNA-binding proteins such as heterogeneous nuclear ribonucleoproteins (hnRNPs) and small nuclear ribonucleoproteins (snRNPs). Methylates NAB2, NPL3, HRP1 and YRA1, shuttling hnRNPs involved in mRNA processing and export, facilitating their export out of the nucleus. Methylation of NPL3 weakens its interaction with THO2, a component of the TREX (transcription/export) complex important for transcriptional elongation and recruitment of mRNA export factors. Methylates the hnRNP HRB1, but does not influence its subcellular location. Methylates the nucleolar proteins GAR1, NOP1 and NSR1. Methylates the snRNP SNP1 and modulates the cotranscriptional recruitment of splicing factors. Dimethylates free histone H4 (HHF1/HHF2) at 'Arg-4' (H4R3me2a) and plays a role in preservation and establishment of silent chromatin domains. Mono- and dimethylates ribosomal protein S2 (RPS2) at 'Arg-11'. Methylates the catalytic subunit of the SWI/SNF chromatin-remodeling complex SNF2.

Gene References into Functions

Data suggest that Hmt1-based arginine methylation is required for Scd6 localization and function. PMID: 27776129
Arginine methylation is a widespread modification, with Hmt1 being the major arginine methyltransferase in S. cerevisiae. PMID: 26572822
the methyltransferase activity of Hmt1 on histone H3R2 requires reciprocal contributions from two Hmt1 molecules, suggesting an intermolecular trans-complementary mechanism by which Hmt1 dimer methylates its substrates PMID: 25715670
Carried out a proteomic profiling experiment to comprehensively identify the physical interactors of Hmt1, the budding yeast homolog for human PRMT1. Snf2 was experimentally confirmed as a robust substrate of Hmt1 in vitro. PMID: 22997150
Interactions between HMT1 and genes encoding components of the histone deacetylase complex Rpd3L, are reported. PMID: 22953000
These results are suggestive of a role for Rmt1 in modifying the function of Rps2 in a manner distinct from that occurring in S. pombe and mammalian cells. PMID: 20035717
Loss of Hmt1's catalytic activity results in dimethylated Arg-3 histone H4 occupancy across silent chromatin regions. PMID: 17158743
Results reveal a functional connection between histone acetylation, methylation, and two of the responsible enzymes, Gcn5p and Hmt1p. PMID: 19358899

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Subcellular Location

Nucleus.

Protein Families

Class I-like SAM-binding methyltransferase superfamily, Protein arginine N-methyltransferase family

Database Links

KEGG: sce:YBR034C

STRING: 4932.YBR034C

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