Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) LIF2 Polyclonal antibody

Uniprot No.

Q9ASP6

Target Names

LIF2

Alternative Names

LIF2 antibody; At4g00830 antibody; A_TM018A10.14 antibody; T18A10.13Heterogeneous nuclear ribonucleoprotein Q antibody; hnRNP Q antibody; Protein LHP1-INTERACTING FACTOR 2 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana LIF2 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Transcriptional activator that binds DNA on GAGA-like motif and 5'-(C/G)ACGTG(G/T)C(A/G)-3' consensus motif in the promoters of target genes. Component of ribonucleosomes, which are complexes of at least 20 other different heterogeneous nuclear ribonucleoproteins (hnRNP). hnRNP play an important role in processing of precursor mRNA in the nucleus. Required during flower development and for cell fate determination. Acts both as an antagonist and as a promoter of polycomb LHP1 gene regulation activity, depending of target genes, to regulate the transcription of stress-responsive and flowering genes. May regulate histone H3 trimethylation on lysine 27 (H3K27me3). Recognizes and binds histone H3 tails methylated at 'Lys-4' (H3K4me) and acetylated at 'Lys-9' (H3K9ac), leading to epigenetic activation. When in complex with LHP1, recognizes and binds histone H3 tails methylated at 'Lys-4' (H3K4me) and 'Lys-27' (H3K27me), mostly corresponding to stress-responsive genes. May function as a suppressor of cell-autonomous immune responses involving glucosinolates, salicylic acid (SA) and jasmonic acid (JA) pathways toward pathogenic bacteria and fungi.

Gene References into Functions

LIF2 may function as a suppressor of cell-autonomous immunity. [LIF2] PMID: 24914891
LIF2, involved in cell identity and cell fate decision, may modulate the activity of LHP1 at specific loci, during specific developmental windows or in response to environmental cues that control cell fate determination. [LIF2] PMID: 21304947

Subcellular Location

Nucleus. Cytoplasm. Microsome.

Tissue Specificity

Predominantly expressed in vascular and meristematic tissues. Expressed throughout development in seedlings, roots, leaves, floral buds and siliques.

Database Links

KEGG: ath:AT4G00830

STRING: 3702.AT4G00830.1

UniGene: At.24279

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LIF2 Antibody

Product Details

Customer Reviews and Q&A

Target Background