Product Details

Full Product Name

Rabbit anti-Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) sty1 Polyclonal antibody

Uniprot No.

Q09892

Target Names

sty1

Alternative Names

sty1 antibody; hog1 antibody; phh1 antibody; spc1 antibody; SPAC24B11.06c antibody; Mitogen-activated protein kinase sty1 antibody; MAP kinase sty1 antibody; EC 2.7.11.24 antibody; MAP kinase spc1 antibody

Raised in

Rabbit

Species Reactivity

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Immunogen

Recombinant Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) sty1 protein

Immunogen Species

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Mitogen-activated protein kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment. Controls osmotic regulation of transcription of target genes. Involved in osmoregulation and stress response pathways leading to an efficient start of sexual differentiation. Supports translation initiation and facilitates adaptation to environmental stress in part through reducing eIF2-alpha phosphorylation. Links the cell-cycle G2/M control with changes in the extracellular environment that affect cell physiology. Phosphorylates atf1 and mkp1. In conjunction with hal4, has a role in the cellular resistance to toxic cations such as Na(+), Li(+) and Ca(2+). Involved in resistance to arsenite, methylglyoxal and hydrogen peroxide. Involved in induction of thermotolerance in mRNA export, as well as in vacuolar fission.

Gene References into Functions

The fission yeast stress-activated protein kinase Sty1, a homolog of mammalian p38 MAP kinase, regulates localization of the Cdc42 polarity module. PMID: 27746023
Data suggest that osmotic stress induces hyperphosphorylation of mitotic inducer cdr1 for several hours and cells delay division during same time period; this stress-induced hyperphosphorylation requires both cdr1 autophosphorylation and stress-activated protein kinase sty1. (cdr1 = serine/threonine protein kinase cdr1/nim1; sty1 = MAP kinase sty1) PMID: 28924043
Threonine-monophosphorylated Sty1 displays a limited biological activity particularly at the transcriptional level. PMID: 26432170
Data suggests that Sty1 activation promotes Pyp2 phosphorylation to increase the stability of the phosphatase. This MAPK-dependent Pyp2 stabilisation allows cells to attenuate MAPK signalling and resume cell division, once stresses have been dealt with. PMID: 23690545
Data indicate that MAPKKK heteromer promotes stress signaling to the Spc1 MAPK. PMID: 23389634
Activation of Sty1 and Atf1 affect chromatin architecture of a large fraction of the Schizosaccharomyces pombe genome to trigger the cellular response to environmental stress.[Review] PMID: 21992435
MAP kinase kinase kinase (MAPKKK)-dependent and -independent activation of Sty1 stress MAPK in fission yeast. PMID: 20729203
Sty1 recruitment primarily occurs at the Atf1/Pcr1 binding sites and is induced by H(2)O(2). PMID: 20661279
Data reveal that redox regulation of cysteine thiols in Sty1 is involved in a stress-specific mechanism regulating transcriptional responses to oxidative stress. PMID: 20061379
These findings point to a regulatory coordination between the Sty1 MAPK and Hri1/Hri2 eIF2alpha kinase pathways for a particular range of stress responses. PMID: 19880757
In response to stress, Sty1/Spc1 phosphorylates Srk1 at threonine 463 of the regulatory domain, inducing both activation of Srk1 kinase, which negatively regulates cell cycle progressio. PMID: 18272791
Has an evolutionary conserved function in the regulation of lipid droplet metabolism. PMID: 18612143
The Spc1 protein kinase regulates the pathway of Atf1-promoted recombination at or before the point where Atf1 binds to chromosomes, and this pathway regulation is independent of the phosphorylation status of Atf1. PMID: 19436749
The SAPK Spc1/Sty1 is essential for cellular resistance to Na+, Li+, and Ca2+. It positively regulates expression of Sod2, a plasma membrane Na+/H+ antiporter. Spc1/Sty1 and the protein kinase Hal4 act cooperatively for cellular cation homeostasis. PMID: 15870269

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Subcellular Location

Cytoplasm. Nucleus. Note=Predominantly cytoplasmic in unstressed cells but rapidly concentrates within the nucleus in response to hyperosmotic conditions and phosphorylation.

Protein Families

Protein kinase superfamily, Ser/Thr protein kinase family, MAP kinase subfamily. HOG1 sub-subfamily

Database Links

KEGG: spo:SPAC24B11.06c

STRING: 4896.SPAC24B11.06c.1

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sty1 Antibody

Product Details

Customer Reviews and Q&A

Target Background