hpf Antibody

Datasheet

Code	CSB-PA360336XA01ENV
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Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) hpf Polyclonal antibody

Uniprot No.

P0AFX0

Target Names

hpf

Alternative Names

hpf antibody; yhbH antibody; b3203 antibody; JW3170 antibody; Ribosome hibernation promoting factor antibody; HPF antibody; Hibernation factor HPF antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) hpf protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

During stationary phase, promotes and stabilizes dimerization of 70S ribosomes by the ribosome modulation factor (RMF), leading to the formation of inactive 100S ribosomes. Converts immature 90S particles formed by RMF into 100S ribosomes. Crystallization with T.thermophilus 70S ribosomes shows it binds in the channel between the head and body of the 30S subunit, where mRNA, tRNAs, initiation factors IF1 and IF3 and elongation factor G would bind; however RMF is still able to bind. In this crystal binding of HPF induces movement of the 30S head domain away from the rest of the ribosome, presumably so they would more easily form dimers. May also function as a potential translational inhibitor.

Gene References into Functions

Since 100S particles were seen in the yfiA deletion mutant containing RMF and YhbH, YhbH probably converts immature 90S ribosomes into mature 100S particles. PMID: 16324148
the first solution structure of HPF as determined by multi-dimensional NMR was reported. PMID: 19747895

Protein Families

HPF/YfiA ribosome-associated protein family, Short HPF subfamily

Database Links

KEGG: ecj:JW3170

STRING: 316385.ECDH10B_3377

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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hpf Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background