Hsf Antibody

Code CSB-PA338468XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) Hsf Polyclonal antibody
Uniprot No.
Target Names
Hsf
Alternative Names
Hsf antibody; CG5748 antibody; Heat shock factor protein antibody; HSF antibody; Heat shock transcription factor antibody; HSTF antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) Hsf protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
Gene References into Functions
  1. Azadiradione ameliorates polyglutamine expansion disease in Drosophila by potentiating DNA binding activity of heat shock factor 1. PMID: 27835876
  2. HSF role in the heat shock response to viral infection PMID: 26234525
  3. HSF is dispensable for establishing or maintaining Pol II pausing but is critical for the release of paused Pol II into the gene body at a subset of highly activated genes PMID: 27492368
  4. The survival curves of heterozygotes on hsf-1 and hsf-4 alleles are similar in spite of HSF-4 protein is slightly active on normal temperature. PMID: 26856085
  5. Physiological function of mutated HSF4 is not fully abrogated after heat exposure and is able to recover even after severe heat stress, causing the induction of heat-shock gene expression. PMID: 25850300
  6. HSF may be regulating many genes besides the known HS genes and that some of these genes may be regulated during non-stress conditions PMID: 21264254
  7. HSF binds to nmnat promoter and induces NMNAT expression under heat shock. In contrast, under hypoxia, HIF1alpha up-regulates NMNAT indirectly through the induction of HSF PMID: 21478149
  8. Recruitment of the master regulator, HSF, is first detected within 20 s of gene activation. PMID: 21172661
  9. The genome-wide distribution of Drosophila Heat Shock Factor (HSF), a transcription factor whose binding activity is mediated by heat shock-induced trimerization, was examined. PMID: 20844575
  10. Three new alternatively spliced isoforms of Drosophila HSF (dHSF) mRNA, named dHSFb, dHSFc, and dHSFd were identified. PMID: 15978579
  11. heat shock factor transcription is up-regulated during hypoxia due to direct binding by hypoxia-inducible factor-1 PMID: 17040902
  12. Life span alteration after irradiation in Drosophila melanogaster strains with mutations of Hsf and Hsps. PMID: 18551381
  13. HSF regulates the expression of several uncharacterized genes induced by a short non-lethal heat treatment:cg-13227,-13664,-32130. cg-6332 & 18331 did not seem to require HSF> PMID: 18759003

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Subcellular Location
Nucleus.
Protein Families
HSF family
Database Links

KEGG: dme:Dmel_CG5748

UniGene: Dm.4107

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