Product Details

Full Product Name

Rabbit anti-Caenorhabditis elegans daf-21 Polyclonal antibody

Uniprot No.

Q18688

Target Names

daf-21

Alternative Names

daf-21 antibody; C47E8.5 antibody; Heat shock protein 90 antibody; Abnormal dauer formation protein 21 antibody

Raised in

Rabbit

Species Reactivity

Caenorhabditis elegans

Immunogen

Recombinant Caenorhabditis elegans daf-21 protein

Immunogen Species

Caenorhabditis elegans

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. In response to cellular stress, up-regulated in distal tissues in a pqm-1-dependent manner, preventing protein misfolding and maintaining proteostasis. By stabilizing the receptor-type guanylate cyclase daf-11 or another signal transduction component that regulates cGMP levels, plays a role in dauer formation and chemotaxis to non-volatile and volatile attractants detected by AWC sensory neurons. Participates in the control of cell cycle progression at the prophase/metaphase transition in oocyte development by ensuring the activity of wee-1.3 kinase, which negatively regulates cdk-1 through its phosphorylation. Regulates yap-1 nuclear export after heat shock treatment.

Gene References into Functions

The study shows that a conserved tryptophan in the middle domain senses the interaction of Hsp90 with a stringent client protein and transfers this information via a cation-pi interaction with a neighboring lysine. PMID: 29662162
DAF-21 Ienterd this protein as newentry study explores the role and contribution of MAP Kinase pathway and its regulator protein DAF-21 involvement in the immunity against opportunistic pathogen P. mirabilis infection PMID: 27072109
The activity of protein phosphatase 5 towards native clients, such as glucocorticoid receptor, is modulated by the middle- and C-terminal domains of Hsp90. PMID: 26593036
Suppression of misfolding in muscle cells is achieved not only by enhanced expression of HSP90 in muscle cells but also by elevated expression of HSP90 (Daf-21) in intestine or neuronal cells. This cell-nonautonomous control of HSP90 expression relies upon transcriptional feedback between somatic tissues that is regulated by the FoxA transcription factor PHA-4. PMID: 23746847
Hsp90 (DAF-21) appears to participate in the maintenance of muscle structures as a transiently associated diffusible factor PMID: 21980476
DAF-21 indirectly regulates the meiotic prophase/metaphase transition during oocyte development by ensuring the normal function of WEE-1.3. PMID: 16466390
PA28 is likely to function in collaboration with Hsp90. PMID: 16650828

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Subcellular Location

Cytoplasm, perinuclear region. Note=Perinuclear region of somatic cells.

Protein Families

Heat shock protein 90 family

Tissue Specificity

In the embryo comma stage, expression is strongly detected in cells of the head region and less so in other areas. In early larvae, expressed in postembryonic germ cells derived from Z2 and Z3 cells and the head region, in both hermaphrodites and males. U

Database Links

KEGG: cel:CELE_C47E8.5

STRING: 6239.C47E8.5.4

UniGene: Cel.17789

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daf-21 Antibody

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