Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) HST1 Polyclonal antibody

Uniprot No.

P53685

Target Names

HST1

Alternative Names

HST1 antibody; YOL068C antibody; NAD-dependent protein deacetylase HST1 antibody; EC 2.3.1.286 antibody; Homologous to SIR2 protein 1 antibody; Regulatory protein SIR2 homolog 1 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) HST1 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

NAD-dependent histone deacetylase involved in telomeric silencing. Histone deacetylase proteins act via the formation of large multiprotein complexes that are responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Restores silencing at HMR in SIR2 mutants when overexpressed. Required to repress middle sporulation genes during vegetative growth. Acts as a sensor of NAD(+) levels and regulator of NAD(+) biosynthesis. Regulates the gene expression of de novo NAD(+) biosynthesis genes.

Gene References into Functions

study demonstrates that Hst1, the closest Sir2 paralogue, deacetylates the acetylated lysine 16 of histone H4 (H4K16Ac) and represses PMA1 transcription in the sir2Delta pde2Delta mutant. Collectively,results suggest that Hst1 can substitute for Sir2 by deacetylating H4K16Ac only in the sir2Delta pde2Delta PMID: 28120189
Crystals of HST1 grown by the hanging-drop vapour-diffusion method diffracted to 2.90 A resolution and belonged to space group P2(1), with unit-cell parameters a = 40.2, b = 101.7, c = 43.9 A, beta = 103.9 degrees PMID: 22139171
Data show that multiple thiamine (THI) genes in Saccharomyces cerevisiae are also regulated by the intracellular NAD(+) concentration via the NAD(+)-dependent histone deacetylase (HDAC) Hst1 and, to a lesser extent, Sir2. PMID: 20439498
Sir2p and Hst1p are paralogs with non-overlapping functions that can provide genetic robustness against null mutations through a substitution mechanism. PMID: 17676954
The results of this study suggest recruitment of the Sum1/Rfm1/Hst1 complex to a number of yeast origins, where Hst1 deacetylated H4 K5. PMID: 18990212

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Subcellular Location

Nucleus. Note=But not nucleolar, and cytoplasmic.

Protein Families

Sirtuin family, Class I subfamily

Database Links

KEGG: sce:YOL068C

STRING: 4932.YOL068C

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HST1 Antibody

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