HST2 Antibody

Code CSB-PA345697XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) HST2 Polyclonal antibody
Uniprot No.
Target Names
HST2
Alternative Names
HST2 antibody; YPL015C antibody; LPA2CNAD-dependent protein deacetylase HST2 antibody; EC 2.3.1.286 antibody; Homologous to SIR2 protein 2 antibody; Regulatory protein SIR2 homolog 2 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) HST2 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
NAD-dependent histone deacetylase that is involved in nuclear silencing events. Derepresses subtelomeric silencing and increases repression in nucleolar (rDNA) silencing. Its function is negatively regulated by active nuclear export.
Gene References into Functions
  1. results show that HST2 is responsible for Sir2-independent life-span extension by calorie restriction and that it does so by suppressing rDNA recombination, the same mechanism by which SIR2 extends life span PMID: 16051752
  2. Here, we show that Hst2 moves between the nucleus and cytoplasm, but is largely cytoplasmic owing to efficient nuclear export.Disruption of Hst2 export shows that nuclear exclusion inhibits the activity of Hst2 as a transcriptional repressor. PMID: 17110954
  3. Specific functions were found for HST2. PMID: 17446861
Subcellular Location
Cytoplasm. Nucleus. Note=Shuttles between the nucleus and cytoplasm, but is largely cytoplasmic owing to efficient nuclear export. Nuclear exclusion is mediated by the exportin CRM1.
Protein Families
Sirtuin family, Class I subfamily
Database Links

KEGG: sce:YPL015C

STRING: 4932.YPL015C

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