HT1 Antibody

Datasheet

Code	CSB-PA646662XA01DOA
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Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) HT1 Polyclonal antibody

Uniprot No.

Q2MHE4

Target Names

HT1

Alternative Names

HT1 antibody; SUU antibody; At1g62400 antibody; F24O1.13 antibody; Serine/threonine/tyrosine-protein kinase HT1 antibody; EC 2.7.10.1 antibody; EC 2.7.11.1 antibody; High leaf temperature protein 1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana HT1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Serine/threonine/tyrosine kinase involved in the control of stomatal movement in response to CO(2). Functions as a major negative regulator of CO(2)-induced stomatal closing. Does not seem to be involved in stomatal closure in response to abscisic acid (ABA) or light. Involved in the control of red light-induced stomatal opening. Is epistatic to SRK2E/OST1 function during stomatal responses to red light and altered CO(2). Phosphorylates SRK2E/OST1 and GHR1 to prevents SRK2E/OST1- and GHR1-induced activation of SLAC1, thus preventing stomatal closure.

Gene References into Functions

Both the loss-of-function and gain-of-function ht1 mutants have completely disrupted CO2 responses, although they have normal responses to ABA. Light-induced stomatal opening is smaller in ht1-3 allele and much smaller in ht1-2. The results indicate that HT1 is a critical regulator for CO2 signaling and is partially involved in the light-induced stomatal opening pathway. PMID: 27034327
These results demonstrate that HT1 is essential for red light-induced stomatal opening and interacts genetically with OST1 during stomatal responses to red light and altered [CO2 ]. PMID: 26192339

Subcellular Location

Cell membrane.

Protein Families

Protein kinase superfamily, Ser/Thr protein kinase family

Tissue Specificity

Mainly localizes in guard cells. Expressed at low level in leaves, stems, roots and flowers.

Database Links

KEGG: ath:AT1G62400

STRING: 3702.AT1G62400.1

UniGene: At.28069

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Protein Service

Gene Synthesis Service

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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HT1 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background