rqh1 Antibody

Code CSB-PA277668XA01SXV
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Product Details

Full Product Name
Rabbit anti-Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) rqh1 Polyclonal antibody
Uniprot No.
Target Names
rqh1
Alternative Names
rqh1 antibody; hus2 antibody; rad12 antibody; rec9 antibody; SPAC2G11.12 antibody; ATP-dependent DNA helicase hus2/rqh1 antibody; EC 3.6.4.12 antibody
Raised in
Rabbit
Species Reactivity
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Immunogen
Recombinant Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) rqh1 protein
Immunogen Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Has a role in the repair of UV-induced DNA damage in G2 via recombination-mediated repair. Also has a role in the repair of infrared-induced double DNA strand breaks. Exhibits an ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction.
Gene References into Functions
  1. During replication-coupled DNA repair, CAF-1 promotes template switch by counteracting D-loop disassembly by Rqh1. PMID: 25313826
  2. Long G2 phase of pot1-rqh1 double mutant may allow time for the accumulation of recombination intermediates which disturb chromosome segregation and make cells sensitive to thiabendazole. PMID: 26093291
  3. Rqh1 is required for the maintenance of circular chromosomes. PMID: 23297345
  4. rqh1 plays a role late in HR following replication arrest and Rhp55/57 has a postsynaptic function PMID: 15802523
  5. Rqh1 acts to prevent blocked replication forks from collapsing and thereby inducing deletion events. PMID: 15889146
  6. a function for Rqh1 in chromosome segregation even in the absence of exogenous insult to the DNA. PMID: 16303848
  7. These observations underscore the multiple recombination functions of RecQ homologs and emphasize that even conserved proteins can be adapted to play different roles in different organisms. PMID: 18562672
  8. Sumoylation of RecQ helicase controls the fate of dysfunctional telomeres. PMID: 19285940

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Subcellular Location
Nucleus.
Protein Families
Helicase family, RecQ subfamily
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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