Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) HXK1 Polyclonal antibody

Uniprot No.

Q42525

Target Names

HXK1

Alternative Names

HXK1 antibody; GIN2 antibody; At4g29130 antibody; F19B15.160 antibody; Hexokinase-1 antibody; EC 2.7.1.1 antibody; Protein GLUCOSE INSENSITIVE 2 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana HXK1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Fructose and glucose phosphorylating enzyme. May be involved in the phosphorylation of glucose during the export from mitochondrion to cytosol. Acts as sugar sensor which may regulate sugar-dependent gene repression or activation. Mediates the effects of sugar on plant growth and development independently of its catalytic activity or the sugar metabolism. May regulate the execution of program cell death in plant cells. Promotes roots and leaves growth.

Gene References into Functions

HXK1 affects the expression of PIPaquaporins genes and reduce leaf water conductance, to coordinate sugar levels with the loss of water through transpiration. PMID: 28390076
role in cell death mediated by myo-inositol accumulation PMID: 26048869
The structure of HXK1 was determined both in its inactive unliganded form and in its active glucose-bound form at resolutions of 1.8 and 2.0 A, respectively. PMID: 25664748
The CO2 metabolism of the gin2-1 mutation in A. thaliana is reported. PMID: 25482780
HXK1 mediated stimulation of NRT2.1 expression. PMID: 24272701
Data suggest that regulation of NIA1 and NIA2 nitrate reductases (NR) activity by sugars does not involve HXK1 as a glucose sensor. PMID: 23480350
Increased expression of HXK in guard cells accelerates stomatal closure. PMID: 23738737
Overexpression of AtHXK1 inhibits growth and accelerates leaf senescence under normal growth conditions. PMID: 22451715
HKL1 and HXK1 can interact, these two proteins likely form a critical node in Glc signaling that mediates overlapping, but also distinct, cellular responses to Glc and ethylene treatments. PMID: 22366209
HXK1-dependent glucose signaling uncoupled from other plant stress and defense hormone signaling has a central regulatory role in early seedling establishment. PMID: 20034964

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Subcellular Location

Mitochondrion outer membrane; Single-pass membrane protein. Nucleus.

Protein Families

Hexokinase family

Tissue Specificity

Highly expressed in flowers and siliques, at intermediate levels in roots and stems, and at lower levels in rosette and cauline leaves.

Database Links

KEGG: ath:AT4G29130

STRING: 3702.AT4G29130.1

UniGene: At.24726

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HXK1 Antibody

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