hypF Antibody

Code CSB-PA329968XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) hypF Polyclonal antibody
Uniprot No.
Target Names
hypF
Alternative Names
hypF antibody; hydA antibody; b2712 antibody; JW5433Carbamoyltransferase HypF antibody; EC 6.2.-.- antibody; Carbamoyl phosphate-converting enzyme HypF antibody; [NiFe]-hydrogenase maturation factor HypF antibody; Hydrogenase maturation protein HypF antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) hypF protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in the maturation of [NiFe] hydrogenases. Along with HypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases. HypF functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carboxamido moiety in an ATP-dependent reaction to the thiolate of the C-terminal cysteine of HypE yielding a protein-S-carboxamide. In the absence of any other substrate, displays carbamoyl-phosphate phosphatase activity.
Gene References into Functions
  1. These findings demonstrate that it is possible to isolate incompletely processed intermediates on the maturation pathway and to use these to activate apo-forms of [NiFe]-hydrogenase large subunits. PMID: 25184670
  2. Data support model in which HypB-SlyD complex establishes communication between GTP hydrolysis and nickel delivery and provide insight into role of HypB-SlyD complex during [NiFe]-hydrogenase biosynthesis. PMID: 22085337
  3. structural flexibility of HypF-N protein and hydrophobic exposure are primary determinants of the ability of oligomeric assemblies to cause cellular dysfunction and its consequences, such as neurodegeneration. PMID: 20081829
  4. Data indicate that HypF-N is capable of forming amyloid protofibrils and fibrils under conditions in which the protein is initially in a predominantly native-like conformation. PMID: 15740744
  5. Experiments reveal that HypF and HypE associate to form a stoichiometric, hetero-oligomeric complex predominantly consisting of a [EF]2 heterotetramer which exists in a dynamic equilibrium with the EF heterodimer. PMID: 18065529

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Protein Families
Carbamoyltransferase HypF family
Database Links
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