Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) IBH1 Polyclonal antibody

Uniprot No.

Q9SKX1

Target Names

IBH1

Alternative Names

IBH1 antibody; BHLH158 antibody; At2g43060 antibody; MFL8.8 antibody; Transcription factor IBH1 antibody; AtIBH1 antibody; BHLH transcription factor zeta antibody; bHLH zeta antibody; Basic helix-loop-helix protein 158 antibody; AtbHLH158 antibody; bHLH 158 antibody; Protein ILI1-BINDING BHLH 1 antibody; bHLH transcription factor bHLH158 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana IBH1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Atypical and probable non DNA-binding bHLH transcription factor that acts as transcriptional repressor that negatively regulates cell and organ elongation in response to gibberellin (GA) and brassinosteroid (BR) signaling. Is able to form heterodimer with BHLH49, thus inhibiting DNA binding of BHLH49, which is a transcriptional activator that regulates the expression of a subset of genes involved in cell expansion by binding to the G-box motif. Binds and inhibits HBI1, a positive regulator of cell elongation that directly binds to the promoters and activated the two expansin genes EXPA1 and EXPA8, encoding cell wall loosening enzymes. The ability of IBH1 to inhibit BHLH49 and HBI1 is counteracted by binding to the antagonist bHLH transcription factor PRE1, restoring the transcriptional activity of BHLH49 and HBI1 and resulting in induction of cell elongation. Functions redundantly with IBL1/BHLH159 in a regulation node known as the incoherent feed-forward loop (FFL).

Gene References into Functions

with BEE contributes to control tolerance to abiotic stress PMID: 29650152
HBI1 interacts with IBH1 which likely keep HBI1 under negative control. PMID: 24443525
IBH1 repressed direct IBL1 transcription, and they both acted in tandem to suppress the expression of a common downstream helix-loop-helix (HLH)/bHLH network, thus forming an incoherent feed-forward loop. PMID: 24505057
The expression of IBH1 and PRE1 is regulated by brassinosteroid, gibberellins, and developmental phase dependent factors, indicating that two phytohormones and phase-dependent signals are integrated by this triantagonistic bHLH system. PMID: 23161888
PRE1-AtIBH1 is a conserved pair of antagonistic HLH/bHLH transcription factors that function downstream of BZR1 to mediate brassinosteroid regulation of cell elongation. [IBH1] PMID: 20009022

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Subcellular Location

Nucleus.

Protein Families

BHLH protein family

Tissue Specificity

Highly expressed in roots and at lower levels in rosette leaves, stems and cauline leaves.

Database Links

KEGG: ath:AT2G43060

STRING: 3702.AT2G43060.1

UniGene: At.27337

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IBH1 Antibody

Product Details

Customer Reviews and Q&A

Target Background