ICME Antibody

Code CSB-PA846409XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ICME Polyclonal antibody
Uniprot No.
Target Names
ICME
Alternative Names
ICME; PCME; At5g15860; F14F8.240; Isoprenylcysteine alpha-carbonyl methylesterase ICME; Isoprenylcysteine methylesterase; Prenylcysteine methylesterase; AtPCME
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana ICME protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Catalyzes the demethylation of isoprenylcysteine methylesters. In vitro, is specific for N-acetyl-S-farnesyl-L-cysteine methyl ester (AFCme) and has low activity toward N-acetyl-S-geranyl-L-cysteine methyl ester (AGCme). Acts as a positive regulator of ABA signaling. May be involved in the demethylation and inactivation of isoprenylated negative regulators of abscisic acid (ABA) signaling. Carboxyl methylation is a reversible and potentially regulated step in the post-translational modification of prenylated proteins.
Gene References into Functions
  1. At5g15860 gene confers prenylcysteine alpha-carboxyl methylesterase (PCME) activity in Arabidopsis thaliana membranes. PMID: 16870359
Subcellular Location
Endoplasmic reticulum membrane. Golgi apparatus membrane; Multi-pass membrane protein.
Protein Families
AB hydrolase superfamily, Isoprenylcysteine methylesterase family
Tissue Specificity
Expressed in roots, rosette and cauline leaves, stems, flowers and siliques.
Database Links

KEGG: ath:AT5G15860

STRING: 3702.AT5G15860.1

UniGene: At.10166

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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