Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) IDN2 Polyclonal antibody

Uniprot No.

Q8VZ79

Target Names

IDN2

Alternative Names

IDN2 antibody; RDM12 antibody; At3g48670 antibody; T8P19.180Protein INVOLVED IN DE NOVO 2 antibody; Protein RNA-DIRECTED DNA METHYLATION 12 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana IDN2 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Forms a complex with FDM1/IDNL1 and FDM2/IDNL2 that is required for RNA-directed DNA methylation (RdDM) and that functions at a downstream step of the RdDM pathway and downstream of small interfering RNA (siRNA) formation. Required for de novo DNA methylation, siRNA accumulation and siRNA-mediated maintenance methylation. Required for several post-transcriptional gene silencing pathways. Binds double-stranded RNAs (dsRNAs) with 5'-overhangs through its XS domain. Binds long non-coding RNA (lncRNA) in an AGO4-dependent manner and associates with DRM2, resulting in DNA methylation of RdDM target loci. Mediates the silencing of a subset of MORC6 target loci.

Gene References into Functions

FDM1 interacts with itself and with IDN2. The XH domain of FDM1 mediates the FDM1-FDM1 and FDM1-IDN2 interactions. PMID: 22757778
The paper presents a refined model for the pathway of RNA-directed DNA methylation and transcriptional gene silencing that positions function of IDN2 downstream of siRNA formation and points to an important role for its XH domain. PMID: 22810086
we demonstrated that the previously uncharacterized IDN2 XH domain is required for the IDN2-IDP1/IDP2 complex formation as well as for IDN2 function. IDP1 is required for de novo DNA methylation, siRNA accumulation, and transcriptional gene silencing. PMID: 22570638
Data show that two partially redundant proteins termed IDN2-LIKE 1 (IDNL1; AT1G15910) and IDN2-LIKE 2 (IDNL2; AT4G00380) that are paralogous to INVOLVED IN DE NOVO 2 (IDN2) and that form a stable complex with IDN2 in vivo. PMID: 22592791
IDN1 (DMS3) and IDN2 control de novo methylation and small interfering RNA (siRNA)-mediated maintenance methylation and are components of the RNA-directed DNA methylation pathway. [IDN2] PMID: 19915591
RDM12 encodes an SGS3-like protein, and is a component of the RNA-directed DNA methylation pathway. [RDM12] PMID: 20059743

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Database Links

KEGG: ath:AT3G48670

STRING: 3702.AT3G48670.1

UniGene: At.27894

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IDN2 Antibody

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