eif-2beta Antibody

Code CSB-PA631368XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans eif-2beta Polyclonal antibody
Uniprot No.
Target Names
eif-2beta
Alternative Names
K04G2.1Eukaryotic translation initiation factor 2 subunit 2 antibody; Eukaryotic translation initiation factor 2 subunit beta antibody; eIF-2-beta antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans eif-2beta protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
Gene References into Functions
  1. Isolation and characterization of mutations in eif-2Ba encoding the a-subunit of eIF2B support a conserved role for eIF2Balpha in providing a binding site for phosphorylated eIF2a to inhibit the exchange factor eIF2B catalytic activity that is required for translation initiation PMID: 28292919
  2. These analyses further support the critical role of eIF2beta in start codon selection. PMID: 20215469
Protein Families
EIF-2-beta/eIF-5 family
Database Links

KEGG: cel:CELE_K04G2.1

STRING: 6239.K04G2.1.3

UniGene: Cel.18516

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