Product Details

Full Product Name

Rabbit anti-Oryza sativa subsp. japonica (Rice) EIF4A3A Polyclonal antibody

Uniprot No.

Q5VNM3

Target Names

EIF4A3A

Alternative Names

EIF4A3A antibody; EIF4A3 antibody; RH2 antibody; Os01g0639100 antibody; LOC_Os01g45190 antibody; OsJ_02769 antibody; P0004A09.21 antibody; P0696E01.42Eukaryotic initiation factor 4A-III homolog A antibody; OseIF4AIIIa antibody; eIF-4A-III antibody; eIF4A-III antibody; EC 3.6.4.13 antibody; DEAD-box ATP-dependent RNA helicase 2 antibody; OsRH2 antibody

Raised in

Rabbit

Species Reactivity

Oryza sativa subsp. japonica (Rice)

Immunogen

Recombinant Oryza sativa subsp. japonica (Rice) EIF4A3A protein

Immunogen Species

Oryza sativa subsp. japonica (Rice)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

ATP-dependent RNA helicase. Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Its RNA-dependent ATPase and RNA-helicase activities are induced by MLN51/CASC3, but abolished in presence of the MAGO-Y14 heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGO-Y14 heterodimer increases the RNA-binding affinity of the EJC. EJC core proteins play essential roles in rice development, growth and reproduction. Regulates the splicing of UDT1 (UNDEVELOPED TAPETUM 1) pre-mRNA transcript. UDT1 is a key regulator in stamen development.

Gene References into Functions

Data show that the gene expression patterns OF DEAD box RNA helicases OsRH2 and OsRH34 were similar in various tissues, but the level of OsRH2 mRNA was about 58-fold higher than that of OsRH34 mRNA in seedlings. PMID: 27071313

Subcellular Location

Nucleus. Cytoplasm.

Protein Families

DEAD box helicase family, DDX48/FAL1 subfamily

Tissue Specificity

Expressed in roots, leaves, flowers and seeds.

Database Links

KEGG: osa:4326580

STRING: 39947.LOC_Os01g45190.1

UniGene: Os.39050

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EIF4A3A Antibody

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