EIF4A3B Antibody

Code CSB-PA608871XA01OFG
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Product Details

Full Product Name
Rabbit anti-Oryza sativa subsp. japonica (Rice) EIF4A3B Polyclonal antibody
Uniprot No.
Target Names
EIF4A3B
Alternative Names
EIF4A3B antibody; EIF4A3 antibody; RH34 antibody; Os03g0566800 antibody; LOC_Os03g36930 antibody; OSJNBa0026A15.3Eukaryotic initiation factor 4A-III homolog B antibody; OseIF4AIIIb antibody; eIF-4A-III antibody; eIF4A-III antibody; EC 3.6.4.13 antibody; DEAD-box ATP-dependent RNA helicase 34 antibody; OsRH34 antibody
Raised in
Rabbit
Species Reactivity
Oryza sativa subsp. japonica (Rice)
Immunogen
Recombinant Oryza sativa subsp. japonica (Rice) EIF4A3B protein
Immunogen Species
Oryza sativa subsp. japonica (Rice)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
ATP-dependent RNA helicase. Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Its RNA-dependent ATPase and RNA-helicase activities are induced by MLN51/CASC3, but abolished in presence of the MAGO-Y14 heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGO-Y14 heterodimer increases the RNA-binding affinity of the EJC. EJC core proteins play essential roles in rice development, growth and reproduction. Regulates the splicing of UDT1 (UNDEVELOPED TAPETUM 1) pre-mRNA transcript. UDT1 is a key regulator in stamen development.
Subcellular Location
Nucleus. Cytoplasm.
Protein Families
DEAD box helicase family, DDX48/FAL1 subfamily
Tissue Specificity
Expressed in leaves, flowers and seeds.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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