ife-1 Antibody

Datasheet

Code	CSB-PA524295XA01CXY
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Product Details

Full Product Name

Rabbit anti-Caenorhabditis elegans ife-1 Polyclonal antibody

Uniprot No.

O45551

Target Names

ife-1

Alternative Names

ife-1 antibody; F53A2.6 antibody; Eukaryotic translation initiation factor 4E-1 antibody; eIF-4E-1 antibody; eIF4E-1 antibody; eIF-4F 25 kDa subunit antibody; mRNA cap-binding protein antibody

Raised in

Rabbit

Species Reactivity

Caenorhabditis elegans

Immunogen

Recombinant Caenorhabditis elegans ife-1 protein

Immunogen Species

Caenorhabditis elegans

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. All 5 eIF4E proteins bind monomethyl cap structures. Only ife-1, ife-2 and ife-5 bind trimethyl cap structures which result from trans-splicing. Translation of trimethyl cap structure mRNAs may be regulated by intracellular redox state; disulfide bonds change the width and depth of the cap-binding cavity determining selectivity to mRNA caps. Required for progression through meiotic divisions during spermatogenesis and for the production of viable sperm. It is not required during oogenesis.

Gene References into Functions

Altered MAPK activation in oocytes suggests dual roles for IFE-1, both promoting and suppressing oocyte maturation at different stages. PMID: 26542024
A germline-specific isoform of eIF4E (IFE-1) is required for efficient translation of stored mRNAs and maturation of both oocytes and sperm. PMID: 19383718

Subcellular Location

Cytoplasm. Note=When associated with pgl-1.

Protein Families

Eukaryotic initiation factor 4E family

Tissue Specificity

Enriched in the germline from L3 larvae to adults; regions of the gonad undergoing spermatogenesis. Expressed in germ granules (P granules); when associated with pgl-1.

Database Links

STRING: 6239.F53A2.6a

UniGene: Cel.23053

Promotions

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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ife-1 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background