EIF(ISO)4E Antibody

Code CSB-PA515353XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) EIF(ISO)4E Polyclonal antibody
Uniprot No.
Target Names
EIF(ISO)4E
Alternative Names
EIF(ISO)4E antibody; LSP1 antibody; At5g35620 antibody; MJE4.8 antibody; Eukaryotic translation initiation factor isoform 4E antibody; eIF(iso)4E antibody; Protein LOSS OF SUSCEPTIBILITY TO POTYVIRUS 1 antibody; eIF-(iso)4F 25 kDa subunit antibody; eIF-(iso)4F p28 subunit antibody; eIF4Eiso protein antibody; mRNA cap-binding protein antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana EIF(ISO)4E protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Mediates susceptibility to Turnipmosaic potyvirus (TuMV) and Tobacco etch potyvirus (TEV).
Gene References into Functions
  1. Turnip mosaic virus protein linked to the genome (VPg) inhibits cap-dependent translational initiation via binding to eIF(iso)4E. [eIF(iso)4E] PMID: 18582528
  2. Turnip mosaic virus (TuMV), another member of the genus Potyvirus, multiplied in mutant plants lacking eIF4E but not in mutant PMID: 15710407
Protein Families
Eukaryotic initiation factor 4E family
Tissue Specificity
Abundant in floral organs and in young developing tissues.
Database Links

KEGG: ath:AT5G35620

STRING: 3702.AT5G35620.1

UniGene: At.20245

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