Product Details

Full Product Name

Rabbit anti-Mus musculus (Mouse) Eif6 Polyclonal antibody

Uniprot No.

O55135

Target Names

Eif6

Alternative Names

Eif6 antibody; Itgb4bpEukaryotic translation initiation factor 6 antibody; eIF-6 antibody; B4 integrin interactor antibody; CAB antibody; p27(BBP) antibody

Raised in

Rabbit

Species Reactivity

Mus musculus (Mouse)

Immunogen

Recombinant Mus musculus (Mouse) Eif6 protein

Immunogen Species

Mus musculus (Mouse)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. Behaves as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (RACK1)-dependent protein kinase C activity. In tissues responsive to insulin, controls fatty acid synthesis and glycolysis by exerting translational control of adipogenic transcription factors such as CEBPB, CEBPD and ATF4 that have G/C rich or uORF in their 5'UTR. Required for ROS-dependent megakaryocyte maturation and platelets formation, controls the expression of mitochondrial respiratory chain genes involved in reactive oxygen species (ROS) synthesis. Involved in miRNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Required for both miRNA-mediated translational repression and miRNA-mediated cleavage of complementary mRNAs by RISC. Modulates cell cycle progression and global translation of pre-B cells, its activation seems to be rate-limiting in tumorigenesis and tumor growth.

Gene References into Functions

eIF6 heterozygous mice show an increased mortality during viral infection and a reduction of peripheral blood CD4(+) effector memory T cells PMID: 28743432
Taken together, these results indicate that eIF6 may be involved in external mechanical force-mediated murine dermal fibroblast function at least partly through the TGF-beta1/TGFBR1/TGFBR2 pathway. PMID: 27824055
eIF6 relieved fibrosis/cicatrization by inhibiting the generation of VEGF to prevent the overgrowth of blood vessels and production of granulation tissues, and by regulating the MMP-2/TIMP-2 balance to promote ECM degradation and decrease its deposition. PMID: 27220600
data reveal a novel transcriptional regulatory mechanism of eIF6 that acts on facilitating Sp1 recruitment to TGF-beta1 promoter via H2A.Z depletion and thus results in increased TGF-beta1 transcription, which contributes to myofibroblast differentiation. PMID: 26395397
Reduced eIF6 expression negatively impacts on megakaryopoiesis. in eIF6(+/-) cells, the steady-state abundance of mitochondrial respiratory chain complex I-encoding mRNAs is decreased, resulting in decreased reactive oxygen species production. PMID: 26391622
eIF6 controls fatty acid synthesis and glycolysis in a cell autonomous fashion. Translational activation by eIF6 reshapes gene expression with increased levels of lipogenic and glycolytic enzymes. PMID: 26383020
In a murine model of lymphomagenesis, eIF6 depletion leads to a striking increase of survival, without adverse effects. PMID: 25252159
eIF6 is a rate-limiting controller of initiation of translation, able to affect tumorigenesis and tumor growth. PMID: 21665150
role in forming nuclear matrix filaments PMID: 12150946
eIF6 is the first eIF associated with the large 60S subunit that regulates translation in response to extracellular signals PMID: 18784653

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Subcellular Location

Cytoplasm. Nucleus, nucleolus. Note=Shuttles between cytoplasm and nucleus/nucleolus.

Protein Families

EIF-6 family

Tissue Specificity

Detected in bladder, duodenum, liver, esophagus, pancreas, adipose tissue, megakaryocytes and testis with lower levels in muscle (at protein level).

Database Links

KEGG: mmu:16418

STRING: 10090.ENSMUSP00000029142

UniGene: Mm.271674

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Eif6 Antibody

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