ilvC Antibody

Datasheet

Code	CSB-PA356628XA01ENV
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Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) ilvC Polyclonal antibody

Uniprot No.

P05793

Target Names

ilvC

Alternative Names

ilvC antibody; b3774 antibody; JW3747 antibody; Ketol-acid reductoisomerase antibody; NADP(+) antibody; KARI antibody; EC 1.1.1.86 antibody; Acetohydroxy-acid isomeroreductase antibody; AHIR antibody; Alpha-keto-beta-hydroxylacyl reductoisomerase antibody; Ketol-acid reductoisomerase type 2 antibody; Ketol-acid reductoisomerase type II antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) ilvC protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate. Also able to use 2-ketopantoate, 2-ketoisovalerate, 2-ketovalerate, 2-ketobutyrate, 3-hydroxypyruvate, 3-hydroxy-2-ketobutyrate and pyruvate.

Gene References into Functions

Key cofactor specificity determinants are identified and five enzymes constructed with reversed cofactor preference; additional directed evolution generated two enzymes with NADH-dependent catalytic efficiencies greater than wild-type enzymes with NADPH. PMID: 23776225
Ultracentrifugation studies confirm that the enzyme exists as a tetramer in solution, and isothermal titration calorimetry shows that the binding of Mg(2+) decreases, while the binding of NADPH increases the structural rigidity of the enzyme. PMID: 23036858
crystal structure of Escherichia coli ketol-acid reductoisomerase PMID: 15272168
crystal structure of the E. coli enzyme at 2.6 A resolution, the first three-dimensional structure of any bacterial Class II KARI PMID: 16322583

Subcellular Location

Cytoplasm.

Protein Families

Ketol-acid reductoisomerase family

Database Links

KEGG: ecj:JW3747

STRING: 316385.ECDH10B_3963

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Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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ilvC Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background