ilvC Antibody

Code CSB-PA356628XA01ENV
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Product Details

Full Product Name Rabbit anti-Escherichia coli (strain K12) ilvC Polyclonal antibody
Uniprot No. P05793
Target Names ilvC
Alternative Names ilvC antibody; b3774 antibody; JW3747 antibody; Ketol-acid reductoisomerase antibody; NADP(+) antibody; KARI antibody; EC 1.1.1.86 antibody; Acetohydroxy-acid isomeroreductase antibody; AHIR antibody; Alpha-keto-beta-hydroxylacyl reductoisomerase antibody; Ketol-acid reductoisomerase type 2 antibody; Ketol-acid reductoisomerase type II antibody
Raised in Rabbit
Species Reactivity Escherichia coli (strain K12)
Immunogen Recombinant Escherichia coli (strain K12) ilvC protein
Immunogen Species Escherichia coli (strain K12)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method Protein A/G
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB (ensure identification of antigen)
Protocols ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables ① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee ① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time Made-to-order (14-16 weeks)

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Target Background

Function
(From Uniprot)
Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate. Also able to use 2-ketopantoate, 2-ketoisovalerate, 2-ketovalerate, 2-ketobutyrate, 3-hydroxypyruvate, 3-hydroxy-2-ketobutyrate and pyruvate.
Gene References into Functions
  1. Key cofactor specificity determinants are identified and five enzymes constructed with reversed cofactor preference; additional directed evolution generated two enzymes with NADH-dependent catalytic efficiencies greater than wild-type enzymes with NADPH. PMID: 23776225
  2. Ultracentrifugation studies confirm that the enzyme exists as a tetramer in solution, and isothermal titration calorimetry shows that the binding of Mg(2+) decreases, while the binding of NADPH increases the structural rigidity of the enzyme. PMID: 23036858
  3. crystal structure of Escherichia coli ketol-acid reductoisomerase PMID: 15272168
  4. crystal structure of the E. coli enzyme at 2.6 A resolution, the first three-dimensional structure of any bacterial Class II KARI PMID: 16322583
Subcellular Location Cytoplasm.
Protein Families Ketol-acid reductoisomerase family
Database Links

KEGG: ecj:JW3747

STRING: 316385.ECDH10B_3963

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