KAP104 Antibody

Code CSB-PA339935XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) KAP104 Polyclonal antibody
Uniprot No.
Target Names
KAP104
Alternative Names
KAP104 antibody; YBR017C antibody; YBR0224 antibody; Importin subunit beta-2 antibody; Importin-104 antibody; Karyopherin subunit beta-2 antibody; Karyopherin-104 antibody; Transportin antibody; TRN antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) KAP104 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for arginine/glycine-rich nuclear localization signals (rg-NLS) and PY-NLS in cargo substrates. Its predominant cargo substrate seems to be mRNA-binding proteins. Required for nuclear transport of NAB2, HRP1/NAB4 and TFG2. Mediates docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to repeat-containing nucleoporins. The complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, GTP-Ran binding leads to release of the cargo. Efficient GTP-Ran-mediated substrate release requires RNA. The importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
Gene References into Functions
  1. Data hypothesize on a possible function of Kap104p in the localized protein synthesis. PMID: 20305392
  2. The Kap104p supports import for nuclear uptake of H3 and H4. PMID: 15679097
  3. identified three Proline-tyrosine nuclear localization signals regions that are important for binding Kap104p PMID: 18532879
  4. Temporally and spatially coordinated localization of Kap104p is a new mechanism for the asymmetric distribution of protein synthesis in dividing cells. PMID: 19198597
Subcellular Location
Cytoplasm. Nucleus, nuclear pore complex. Nucleus.
Protein Families
Importin beta family, Importin beta-2 subfamily
Database Links

KEGG: sce:YBR017C

STRING: 4932.YBR017C

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