Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PSE1 Polyclonal antibody

Uniprot No.

P32337

Target Names

PSE1

Alternative Names

PSE1 antibody; KAP121 antibody; YMR308C antibody; YM9952.10C antibody; Importin subunit beta-3 antibody; Karyopherin subunit beta-3 antibody; Karyopherin-121 antibody; Protein secretion enhancer 1 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PSE1 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for classical and arginine/glycine-rich nuclear localization signals (cNLS and rg-NLS) in cargo substrates. Its predominant cargo substrate seems to be ribosomal proteins and ribosome biogenesis trans- and cis-acting factors. Required for nuclear transport of YAP1, NOP1 and SOF1. Mediates the nuclear import of histones H3 and H4. Mediates docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to repeat-containing nucleoporins. The complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, GTP-Ran binding leads to release of the cargo. The importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.; Plays a role in protein secretion.

Gene References into Functions

The RanGTP-resistant interactions of Kap121 with the Dam1 complex would allow Kap121 to escort the complex to its destination on kinetochore-associated microtubule ends. PMID: 26947076
Comparison of the nuclear localization signal and RanGTP complexes reveals that RanGTP binding not only occludes the cargo-binding site but also forces Kap121p into a conformation that is incompatible with nuclear localization signal recognition. PMID: 23541588
The PSE1 specifically binds to histones H2A and H2B. PMID: 15679097

Subcellular Location

Cytoplasm. Nucleus.

Protein Families

Importin beta family, Importin beta-3 subfamily

Database Links

KEGG: sce:YMR308C

STRING: 4932.YMR308C

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PSE1 Antibody

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