IME4 Antibody

Code CSB-PA334902XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) IME4 Polyclonal antibody
Uniprot No.
Target Names
IME4
Alternative Names
IME4 antibody; SPO8 antibody; YGL192W antibody; G1337 antibody; N6-adenosine-methyltransferase IME4 antibody; EC 2.1.1.348 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) IME4 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Catalytic component of the MIS complex, a complex that mediates N6-methyladenosine (m6A) methylation on some mRNAs during meiosis and is required for sporulation. M6A, which takes place on the adenosine of 5'-[AG]GAC-3' consensus sites of some mRNAs, is probably required to initiate sporulation. Positive regulator for IME2.
Gene References into Functions
  1. The determination of the role of Ime4 and its targets in mitochondrial biology could probably help in formulating potential cures for the mitochondria-linked rare genetic disorders. PMID: 28975387
  2. we report that IME4 gene deletion causes peroxisomal dysfunction under stationary-phase conditions in Saccharomyces cerevisiae; besides, the ime4Delta cells showed a significant decrease in the expression of the key genes involved in peroxisomal beta-oxidation compared to the wild-type cells PMID: 29043484
Subcellular Location
Cytoplasm. Nucleus, nucleolus. Note=Localizes in the nucleolus during the period of m6A mRNA accumulation.
Protein Families
MT-A70-like family
Database Links

KEGG: sce:YGL192W

STRING: 4932.YGL192W

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