inaD Antibody

Code CSB-PA638752XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) inaD Polyclonal antibody
Uniprot No.
Target Names
inaD
Alternative Names
inaD antibody; CG3504 antibody; Inactivation-no-after-potential D protein antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) inaD protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in the negative feedback regulation of the light-activated signaling cascade in photoreceptors through a calcium-mediated process. Interacts with tetrapeptide ligand located in C-terminal sequence of 3 key components of the visual cascade, tethering them and forming a macromolecular signaling phototransduction complex.
Gene References into Functions
  1. show that INAD PDZ3 binds to the TRP channel tail with a new mechanism. A 15-residue C-terminal tail of TRP forms a b hairpin structure and binds to a surface of INAD PDZ3 outside the canonical target-binding aB/bB groove. Amino acid residues from both strands of the TRP b hairpin are engaged in binding, and thus the INAD PDZ3/TRP interaction is highly specific and with high affinity. PMID: 26853938
  2. Through biochemical and structural studies, study shows that the redox potential of PDZ5 is allosterically regulated by its interaction with another INAD domain, PDZ4. PMID: 21703451
  3. genetic screen of the third chromosome for mutations that result in mislocalization of an INAD-GFP fusion protein PMID: 20404479
  4. study demonstrates conformational switch mechanism for PDZ domain function & suggest INAD behaves more like a dynamic machine rather than a passive scaffold, regulating signal transduction through cycles of conformational change PMID: 17923089
  5. TRP associates with INAD via its last 14 residues to facilitate its regulation by eye-PKC that fine-tunes the visual signaling PMID: 18036153

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Subcellular Location
Cell membrane; Peripheral membrane protein.
Tissue Specificity
Expressed in rhabdomeres of the compound eyes and ocelli.
Database Links

KEGG: dme:Dmel_CG3504

STRING: 7227.FBpp0293662

UniGene: Dm.2964

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