INP1 Antibody

Code CSB-PA191318XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) INP1 Polyclonal antibody
Uniprot No.
Target Names
INP1
Alternative Names
INP1 antibody; At4g22600 antibody; F7K2.180 antibody; Protein INAPERTURATE POLLEN1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana INP1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Required for the formation of pollen surface apertures, which arise by restriction of exine deposition at specific sites. The aperture length depends on the INP1 dosage. Does not play a role in specifying the number or position of apertures. Acts in a sporophytic manner.
Gene References into Functions
  1. NP1 is a late-acting factor involved in keeping specific membrane domains next to the callose wall to prevent formation of exine at these sites. PMID: 28899962
  2. in the absence of male meiosis the ability of INP1 to assemble into lines at the pollen surface is compromised. PMID: 29173018
  3. n the mutants with ectopic apertures, the number and positions of INP1 localization sites change depending on ploidy or ploidy-related cell size and not on INP1 levels, suggesting that sites for aperture formation are specified before INP1 is brought to them. PMID: 27177036
  4. Loss of INP1 leads to the loss of all three apertures in Arabidopsis pollen, and INP1 protein exhibits a unique tripartite localization in developing pollen, indicative of its direct involvement in specification of aperture positions. PMID: 23136373
Subcellular Location
Cytoplasm.
Tissue Specificity
Expressed only in anthers and in pollen. Not detected in other flower tissues, stems, leaves and siliques.
Database Links

KEGG: ath:AT4G22600

STRING: 3702.AT4G22600.1

UniGene: At.65416

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