CWINV1 Antibody

Code CSB-PA670403XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CWINV1 Polyclonal antibody
Uniprot No.
Target Names
CWINV1
Alternative Names
CWINV1 antibody; BFRUCT1 antibody; At3g13790 antibody; MMM17.26 antibody; Beta-fructofuranosidase antibody; insoluble isoenzyme CWINV1 antibody; EC 3.2.1.26 antibody; Cell wall beta-fructosidase 1 antibody; AtbetaFRUCT1 antibody; Cell wall invertase 1 antibody; AtcwINV1 antibody; Sucrose hydrolase 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana CWINV1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Beta-fructofuranosidase that can use sucrose and 1-kestose, and, to a lower extent, neokestose and levan, as substrates, but not inuline.
Gene References into Functions
  1. Enzyme activity measurements, quantitative RT-PCR, and T-DNA insertional mutagenesis have been used to determine that expression of AtcwINV1 is responsible for all induced cwINV activity in mechanically wounded leaves PMID: 16339783
  2. The structure of the Arabidopsis thaliana cell-wall invertase 1 (AtcwINV1; gene accession code At3g13790) is described at a resolution of 2.15 A. PMID: 17139091
  3. This study showed that a D239A mutant of INV1 acted as a 1-fructan exohydrolase, preferentially degrading 1-kestose. PMID: 17873089
  4. E203Q mutation introduced into the catalytic pocket triggers conformational changes that alter the wild type substrate binding. PMID: 17963237
  5. report on different X-ray crystal structures of Arabidopsis thaliana cell-wall invertase 1 (AtcwINV1) mutants with sucrose PMID: 18258263

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Subcellular Location
Secreted, extracellular space, apoplast. Secreted, cell wall.
Protein Families
Glycosyl hydrolase 32 family
Tissue Specificity
Expressed in seedlings, leaves, flowers, and seeds.
Database Links

KEGG: ath:AT3G13790

STRING: 3702.AT3G13790.1

UniGene: At.24308

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