ipaH9.8 Antibody

Code CSB-PA513237XA01SZB
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Product Details

Full Product Name
Rabbit anti-Shigella flexneri ipaH9.8 Polyclonal antibody
Uniprot No.
Target Names
ipaH9.8
Alternative Names
ipaH9.8 antibody; CP0226 antibody; pWR501_0234 antibody; SFLP090 antibody; E3 ubiquitin-protein ligase ipaH9.8 antibody; EC 2.3.2.27 antibody; Invasion plasmid antigen ipaH9.8 antibody; RING-type E3 ubiquitin transferase ipaH9.8 antibody
Raised in
Rabbit
Species Reactivity
Shigella flexneri
Immunogen
Recombinant Shigella flexneri ipaH9.8 protein
Immunogen Species
Shigella flexneri
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. This protein is an E3 ubiquitin ligase that interferes with host's ubiquitination pathway and modulates the acute inflammatory responses, thus facilitating bacterial colonization within the host cell. Interacts with IKBKG (NEMO) and TNIP1 (ABIN-1), a ubiquitin-binding adapter protein, which results in TNIP1-dependent 'Lys-27'-linked polyubiquitination of IKBKG. Consequently, polyubiquitinated IKBKG undergoes proteasome-dependent degradation, which perturbs NF-kappa-B activation during bacterial infection. Uses UBE2D2 (UBCH5B) as an E2 ubiquitin-conjugating enzyme. Mediates polyubiquitination of host U2AF1, leading to its proteasomal degradation.
Gene References into Functions
  1. under non-reducing conditions IpaH9.8 undergoes a domain swap driven by the formation of a disulfide bridge involving the catalytic cysteine and that this dimer is unable to catalyse the ubiquitination of U2AF35 PMID: 20831869
Subcellular Location
Secreted. Host cytoplasm. Host nucleus. Note=Secreted via Mxi-Spa type III secretion system (TTSS), and delivered into the host cytoplasm. Transported into the host nucleus. This transport is independent of cytosolic factors, but dependent on temperature and partly on ATP/GTP.
Protein Families
LRR-containing bacterial E3 ligase family
Database Links

KEGG: sfl:CP0226

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