IPK1 Antibody

Code CSB-PA842418XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) IPK1 Polyclonal antibody
Uniprot No.
Target Names
IPK1
Alternative Names
IPK1 antibody; At5g42810 antibody; MJB21.19 antibody; Inositol-pentakisphosphate 2-kinase antibody; EC 2.7.1.158 antibody; Inositol-1,3,4,5,6-pentakisphosphate 2-kinase antibody; Ins(1,3,4,5,6)P5 2-kinase antibody; AtIPK1 antibody; InsP5 2-kinase antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana IPK1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Phosphorylates Ins(1,3,4,5,6)P5 at position 2 to form Ins(1,2,3,4,5,6)P6 (InsP6 or phytate). Phytate is a regulator of intracellular signaling, a highly abundant animal antinutrient, and a phosphate store in plant seeds. Also phosphorylates Ins(1,3,4,6)P4 and Ins(1,4,5,6)P4 to produce Ins(1,2,3,4,6)P5 and Ins(1,2,4,5,6)P5.
Gene References into Functions
  1. a particular mechanism of Arsenic(V) toxicity existed in atipk1-1 mutant. PMID: 26264234
  2. Expression of the Gle1 variants with enhanced Myo-inositol-1,2,3,4,5,6-hexakisphosphate (InsP(6)) sensitivity rescues the mRNA export defect of the ipk1 (inositol 1,3,4,5,6-pentakisphosphate 2-kinase) InsP(6)-deficient mutant. PMID: 25670768
  3. IPK is indeed a member of the plant terpenoid metabolic network. PMID: 26216978
  4. AtIPK1 has important roles in growth and phosphate (Pi) homeostasis. PMID: 25155524
  5. Conformational changes in inositol 1,3,4,5,6-pentakisphosphate 2-kinase upon substrate binding: role of N-terminal lobe and enantiomeric substrate preference. PMID: 22745128
  6. Report that limited proteolysis has revealed the presence of multiple conformational states in the IPK1 catalytic cycle and propose a model for recognition of IP substrate by IPK1. PMID: 22362712
  7. The isolation and characterization of ATIPK1 is reported. PMID: 16223361

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Protein Families
IPK1 type 2 family
Tissue Specificity
Strongly expressed in leaves and cauline leaves. Weakly expressed in siliques and flowers. In flower, it is expressed in the major organs of developing flower buds. Strongly expressed in sepals, petals, in the male and female organs of immature and mature
Database Links

KEGG: ath:AT5G42810

STRING: 3702.AT5G42810.1

UniGene: At.8456

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