Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) Ir25a Polyclonal antibody

Uniprot No.

E9NA96

Target Names

Ir25a

Alternative Names

Ir25a antibody; CG15627 antibody; Ionotropic receptor 25a antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) Ir25a protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Integral part of various neural sensory systems in the antenna that provide the neural basis for the response to environmental changes in temperature (thermosensation), humidity (hygrosensation) and odor detection. Required for odor-evoked electrophysiological responses in multiple neuron classes in the antenna and is likely to function as part of an olfactory receptor complex with Ir76a and Ir76b. Together with Ir21a and Ir93a, mediates the response of the larval dorsal organ cool cells, a trio of cool-responsive neurons, to cooling and is required for cool avoidance behavior. Required in chordonotal organ neurons for behavioral synchronization to low-amplitude temperature cycles and mediates circadian clock resetting by temperature. Together with Ir40a and Ir93a, mediates the response of the hydrosensory sacculus neurons to changes in relative humidity, and is required for dry detection and humidiy preference behavior.

Gene References into Functions

Mutation in IR25a gene is associated with loss of appetitive behavioral responses to fatty acids. PMID: 29231818
IR25a and IR76b are essential in sour gustatory receptor neurons of females for oviposition preference on acid-containing food. PMID: 28889974
Here, the authors discover Drosophila moist cells and show that they require IR25a and IR93a together with IR68a, a conserved, but orphan ionotropic receptor for hygrosensation. PMID: 28621663
The ionotropic receptors IR40a, IR93a, and IR25a are all required for humidity preference in D. melanogaster. Whereas IR40a is selectively required for hygrosensory responses, IR93a and IR25a mediate both humidity and temperature preference. Consistent with this, the expression of IR93a and IR25a includes thermosensory neurons of the arista. PMID: 27161501
Drosophila IR25a is required for behavioural synchronization to low-amplitude temperature cycles; IR25a is required for temperature-synchronized clock protein oscillations in subsets of central clock neurons PMID: 26580016

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Subcellular Location

Cell membrane; Multi-pass membrane protein. Cell projection, axon. Cell projection, dendrite. Perikaryon. Cell projection, cilium.

Protein Families

Glutamate-gated ion channel (TC 1.A.10.1) family

Tissue Specificity

In the antenna, detected in neurons of the arista and also detected in sacculus neurons which innervate the first and second chambers (at protein level). Throughout the main body of the antenna, expressed in neurons which innervate the coeloconic class of

Database Links

KEGG: dme:Dmel_CG15627

STRING: 7227.FBpp0304756

UniGene: Dm.23844

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Ir25a Antibody

Product Details

Customer Reviews and Q&A

Target Background