Product Details

Full Product Name

Rabbit anti-Caenorhabditis elegans ire-1 Polyclonal antibody

Uniprot No.

Q09499

Target Names

ire-1

Alternative Names

ire-1 antibody; C41C4.4 antibody; Serine/threonine-protein kinase/endoribonuclease ire-1 antibody; Inositol-requiring protein 2) [Includes: Serine/threonine-protein kinase antibody; EC 2.7.11.1); Endoribonuclease antibody; EC 3.1.26.-)] antibody

Raised in

Rabbit

Species Reactivity

Caenorhabditis elegans

Immunogen

Recombinant Caenorhabditis elegans ire-1 protein

Immunogen Species

Caenorhabditis elegans

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices xbp-1 precursor mRNA to produce the mature form which then induces transcription of UPR target genes. Unfolded protein response (UPR) transcriptional activation by ire-1, as well as translational attenuation by pek-1 in a complementary pathway, maintains ER homeostasis. Regulates the transcriptional up-regulation of nucleoside-diphosphatase apy-1 upon ER stress. By activating the UPR pathway during non-lethal hypoxia pre-conditioning, confers adaptive protection to subsequent exposure to hypoxia. ire-1 and pek-1 are redundant genes that control a pathway essential for larval development and survival. Plays a role in the nuclear retention of unspliced mRNAs.

Gene References into Functions

vitamin-D3-induced lifespan extension requires the stress response pathway genes skn-1, ire-1, and xbp-1. PMID: 27783938
Enhanced study of the role of IIS pathway and epigenetic mechanisms that regulate aging may facilitate progressive prevention and treatment of human age-related diseases. PMID: 28101820
IRE-1 has an ancient function as a cytoplasmic sentinel that activates p38 and SKN-1(Nrf2). csteine modifications induced by ROS signals can direct proteins to adopt unexpected functions and may coordinate many cellular processes. PMID: 27540856
IRE-1 is essential for dendrite morphogenesis. Reducing Insulin/IGF1 signaling can restore normal dendrite arborization in the complete absence of functional IRE-1. PMID: 28114319
In conclusion, we identified PEK-1 as a mediator of hormesis in the mev-1 mutant of C. elegans which is induced by simultaneous inhibition of XBP-1 and its splicing activator IRE-1 and mediated through activation of the proteasome. PMID: 27055592
The authors discovered that endoplasmic reticulum stress suppressed the progression of the lethal germline tumor by activating the endoplasmic reticulum stress sensor IRE-1. PMID: 26192965
This implies that ER stress related germline apoptosis can be determined at the organism level, and is a result of active IRE-1 signaling in neurons PMID: 25340700
ER-associated degradation (ERAD) and phagocytosis, loss of ire-1 hinders the clearance of misfolded proteins from the ER as well as the clearance of proteins that were secreted into the pseudocoleom. PMID: 23843615
PDIA6 controls the decay of IRE1alpha signaling via disulfide-dependent association PMID: 24508390
Our data suggest that the negative feedback loops involving the activation of IRE-1-XBP-1 and PEK-1 pathways serve essential roles, not only at the extremes of ER stress, but also in the maintenance of ER homeostasis under physiological conditions. PMID: 22125500
The endoplasmic reticulum-resident IRE-1 kinase is key nutritional sensors that modulate expression of inducible lipases to maintain whole-body energy homeostasis in C. elegans. PMID: 19416714
Increased lifespan by hif-1 or dietary restriction is dependent on the endoplasmic reticulum (ER) stress regulator inositol-requiring protein-1 (IRE-1) and is associated with lower levels of ER stress. PMID: 19461873

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Subcellular Location

Endoplasmic reticulum membrane; Single-pass type I membrane protein.

Protein Families

Protein kinase superfamily, Ser/Thr protein kinase family

Database Links

KEGG: cel:CELE_C41C4.4

STRING: 6239.C41C4.4a

UniGene: Cel.24223

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ire-1 Antibody

Product Details

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Target Background