Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) IRE1A Polyclonal antibody

Uniprot No.

Q9C5S2

Target Names

IRE1A

Alternative Names

IRE1A antibody; IRE1-2 antibody; At2g17520 antibody; MJB20.8 antibody; Serine/threonine-protein kinase/endoribonuclease IRE1a antibody; Endoplasmic reticulum-to-nucleus signaling 1-2 antibody; Inositol-requiring protein 1-2 antibody; AtIRE1-2 antibody; Serine/threonine-protein kinase/endoribonuclease IRE1-2) [Includes: Serine/threonine-protein kinase antibody; EC 2.7.11.1); Endoribonuclease antibody; EC 3.1.26.-)] antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana IRE1A protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices bZIP60 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator which then induces transcription of UPR target genes. Involved in organ growth regulation. Plays a role in plant immunity and abiotic stress responses.

Gene References into Functions

These data suggest that ER stress-activated pathways, led by IRE1 and BI-1, respond to invading potyvirus and potexviruses to restrict virus infection and enable physiological changes enabling plants to tolerate virus assault. PMID: 27578623
An Arabidopsis mutant of a conserved endoplasmic reticulum (ER) stress sensor IRE1 exhibits defects in the auxin response and levels, which supports that the plant IRE1 is required for auxin homeostasis. PMID: 24180465
Arabidopsis AGB1, a subunit of the ubiquitous heterotrimeric GTP-binding protein family, and AtIRE1A/AtIRE1B independently control two essential plant unfolded protein response pathways. PMID: 21914012
observed that the active form of bZIP60 protein detected in the wild-type was missing in ire1a/ire1b PMID: 22355548
Defects in IRE1 enhance cell death and fail to degrade mRNAs encoding secretory pathway proteins in the Arabidopsis unfolded protein response. PMID: 23509268
the IRE1/bZIP60 branch of unfolded protein response is part of the plant response to pathogens for which two AT IRE1 isoforms play only partially overlapping roles; and IRE1 has both bZIP60-dependent and bZIP60-independent functions in plant immunity. PMID: 22359644

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Subcellular Location

Endoplasmic reticulum membrane; Single-pass type I membrane protein.

Protein Families

Protein kinase superfamily, Ser/Thr protein kinase family

Tissue Specificity

Ubiquitous. Detected in the vascular bundles of young plants, leaves, roots, seedlings and in the receptacles of flowers and vascular bundles of the petals.

Database Links

KEGG: ath:AT2G17520

STRING: 3702.AT2G17520.1

UniGene: At.14585

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IRE1A Antibody

Product Details

Customer Reviews and Q&A

Target Background