ISE2 Antibody

Code CSB-PA495132XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ISE2 Polyclonal antibody
Uniprot No.
Target Names
ISE2
Alternative Names
ISE2 antibody; EMB25 antibody; PDE317 antibody; At1g70070 antibody; F20P5.20 antibody; DExH-box ATP-dependent RNA helicase DExH15 chloroplastic antibody; EC 3.6.4.13 antibody; ATP-dependent RNA helicase ISE2 antibody; Protein EMBRYO DEFECTIVE 25 antibody; Protein INCREASED SIZE EXCLUSION LIMIT 2 antibody; Protein PIGMENT DEFECTIVE 317 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana ISE2 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
RNA helicase involved in group II intron splicing. Essential protein required during embryogenesis. Involved in post-transcriptional gene silencing. Modulates the determination of cell fate. Necessary for normal plasmodesmata (PD) development and aperture regulation.
Gene References into Functions
  1. ISE2 depletion causes splicing defects of chloroplasts transcripts containing group II introns. ISE2 directly binds to chloroplast RNA targets in vivo. PMID: 26147377
  2. Increased size exclusion limit2 (ISE2) function affects plasmodesmata structure and function through the regulation of RNA metabolism and consequent gene expression. [ISE2] PMID: 17601829
Subcellular Location
Plastid, chloroplast. Cytoplasmic granule.
Protein Families
DExH box helicase family
Database Links

KEGG: ath:AT1G70070

STRING: 3702.AT1G70070.1

UniGene: At.35348

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