ISA1 Antibody

Code CSB-PA515339XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ISA1 Polyclonal antibody
Uniprot No.
Target Names
ISA1
Alternative Names
ISA1 antibody; At2g39930 antibody; T28M21.9Isoamylase 1 antibody; chloroplastic antibody; AtISA1 antibody; EC 3.2.1.68 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana ISA1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in the trimming of pre-amylopectin chains. Accelerates the crystallization of nascent amylopectin molecules during starch synthesis. ISA1 and ISA2 work exclusively together as a multimeric holoenzyme. ISA1-ISA2 removes preferentially branches that are very close to other branches.
Gene References into Functions
  1. Endogenous starch debranching enzymes ISA1 and ISA2 of Arabidopsis are conserved among diverse groups of plants. PMID: 24642810
  2. Data indicate that analysis of recombinant enzymes showed that Data iindicate that Arabidopsis ISA1 requires ISA2 as a partner for enzymatic function, whereas maize ISA1 was active by itself. PMID: 24027240
  3. The heteromultimeric debranching enzyme involved in starch synthesis in Arabidopsis requires both isoamylase1 and isoamylase2 subunits for complex stability and activity. PMID: 24098685
  4. Data propose that ISA1 function in normal amylopectin synthesis by promoting amylopectin crystallization but conclude that they are not mandatory for starch granule synthesis. PMID: 19074683
Subcellular Location
Plastid, chloroplast.
Protein Families
Glycosyl hydrolase 13 family
Database Links

KEGG: ath:AT2G39930

STRING: 3702.AT2G39930.1

UniGene: At.20831

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