ISA2 Antibody

Code CSB-PA594155XA01OFG
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Product Details

Full Product Name
Rabbit anti-Oryza sativa subsp. japonica (Rice) ISA2 Polyclonal antibody
Uniprot No.
Target Names
ISA2
Alternative Names
ISA2 antibody; Os05g0393700 antibody; LOC_Os05g32710 antibody; OsJ_17304 antibody; OSJNBa0014C03.3 antibody; Isoamylase 2 antibody; chloroplastic antibody; OsISA2 antibody; EC 3.2.1.68 antibody
Raised in
Rabbit
Species Reactivity
Oryza sativa subsp. japonica (Rice)
Immunogen
Recombinant Oryza sativa subsp. japonica (Rice) ISA2 protein
Immunogen Species
Oryza sativa subsp. japonica (Rice)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Starch-debranching enzyme involved in amylopectin biosynthesis in endosperm. Functions by removing excess branches or improper branches that interfere with the formation of double helices of the cluster chains of amylopectin and crystallization of starch. Works together with ISA1 as heterooligomer. The heterooligomer ISA1 and ISA2 possesses higher affinity than the ISA1 homooligomer for various branched polyglucans in vitro, but no marked differences exist in chain preferences for debranching of amylopectin and phytoglycogen between these forms.
Gene References into Functions
  1. The ISA1 homo-oligomer is essential, but not the ISA1-ISA2 oligomer, for starch production in rice endosperm. PMID: 21436381
Subcellular Location
Plastid, chloroplast.
Protein Families
Glycosyl hydrolase 13 family
Tissue Specificity
Highly expressed in developing endosperm and leaves.
Database Links

KEGG: osa:4338695

UniGene: Os.25202

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