ispH Antibody

Code CSB-PA351782XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) ispH Polyclonal antibody
Uniprot No.
Target Names
ispH
Alternative Names
ispH antibody; lytB antibody; yaaE antibody; b0029 antibody; JW00274-hydroxy-3-methylbut-2-enyl diphosphate reductase antibody; HMBPP reductase antibody; EC 1.17.7.4 antibody; 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) ispH protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis. In vitro, can also hydrate acetylenes to aldehydes and ketones via anti-Markovnikov/Markovnikov addition.
Gene References into Functions
  1. work for the first time identified HMBPP to be a cytotoxic intermediate in MEP pathway and demonstrated that balanced activation of IspG and IspH could eliminate accumulation of HMBPP and MECPP and improve isoprenoids production. PMID: 28864262
  2. IspH can hydrate acetylenes to aldehydes and ketones via anti-Markovnikov/Markovnikov addition. PMID: 22948824
  3. a structure-based rationale of the substrate binding mode of ispH, which in combination with mutational studies and theoretical calculation supports a reaction mechanism representing a biological counterpart of the Birch reduction of allylic alcohols. PMID: 19569147
  4. In LytB an unusual [4Fe-4S](2+) cluster is attached to the protein by three conserved cysteines and contains a hexacoordinated iron linked to three sulfurs of the cluster and three additional oxygen or nitrogen ligands. PMID: 19708647
Protein Families
IspH family
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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