ISU1 Antibody

Code CSB-PA524352XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ISU1 Polyclonal antibody
Uniprot No.
Target Names
ISU1
Alternative Names
ISU1 antibody; ISCU1 antibody; At4g22220 antibody; T10I14.50 antibody; Iron-sulfur cluster assembly protein 1 antibody; AtISU1 antibody; AtIscU1 antibody; Protein ISCU-LIKE 1 antibody; NifU-like N-terminal domain-containing protein ISU1 antibody; NifU-like protein ISU1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana ISU1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Scaffold protein for the de novo synthesis of iron-sulfur (Fe-S) clusters within mitochondria, which is required for maturation of both mitochondrial and cytoplasmic [2Fe-2S] and [4Fe-4S] proteins. First, a [2Fe-2S] cluster is transiently assembled on the scaffold protein ISCU (ISU1, ISU2 or ISU3). In a second step, the cluster is released from ISCU, transferred to a glutaredoxin, followed by the formation of mitochondrial [2Fe-2S] proteins, the synthesis of [4Fe-4S] clusters and their target-specific insertion into the recipient apoproteins. Cluster assembly on ISCU depends on the function of the cysteine desulfurase complex NFS1-ISD11, which serves as the sulfur donor for cluster synthesis, the iron-binding protein frataxin as the putative iron donor, and the electron transfer chain comprised of ferredoxin reductase and ferredoxin, which receive their electrons from NADH.
Gene References into Functions
  1. Results suggest that AtHscA2 in cooperation with AtIsu1 and AtHscB play an important role in the regulation of the Fe-S cluster assembly pathway in plant mitochondria. PMID: 25462017
  2. ISU1 has a role in mediating the assembly of iron-sulfur clusters in mitochondria. PMID: 17417719
Subcellular Location
Mitochondrion matrix. Cytoplasm, cytosol.
Protein Families
NifU family
Tissue Specificity
Expressed in roots, stems, leaves, flowers, pollen and siliques.
Database Links

KEGG: ath:AT4G22220

STRING: 3702.AT4G22220.1

UniGene: At.43669

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