JEN1 Antibody

Code CSB-PA339625XA01SVG
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Product Details

Full Product Name Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) JEN1 Polyclonal antibody
Uniprot No. P36035
Target Names JEN1
Alternative Names JEN1 antibody; YKL217WCarboxylic acid transporter protein homolog antibody
Raised in Rabbit
Species Reactivity Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) JEN1 protein
Immunogen Species Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method Protein A/G
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB (ensure identification of antigen)
Protocols ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables ① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee ① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time Made-to-order (14-16 weeks)

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Target Background

Function
(From Uniprot)
Essential to lactate transport.
Gene References into Functions
  1. Saccharomyces cerevisiae can use two transport mechanisms to import lactic acid: a 'saturable' component of the transport, which requires the presence of a functional Jen1p transporter, and a Jen1p independent 'non-saturable' component. PMID: 23790185
  2. Data show that overexpression of Jen1p enables selenite accumulation in yeast compared with a JEN1 knock out strain. PMID: 20861301
  3. The presence of the JEN1(+391) transcript correlated with rapid glucose-triggered mRNA degradation of the JEN1(-51) transcript, whereas when the small transcript started at position +972, the JEN1(-51) mRNA turnover rate was unaffected. PMID: 15896325
  4. Data show that casein kinase 1-dependent phosphorylation and HECT-ubiquitin ligase Rsp5-dependent ubiquitylation are required for Jen1 endocytosis. PMID: 19433580
Subcellular Location Membrane; Multi-pass membrane protein.
Protein Families Major facilitator superfamily, Sugar transporter (TC 2.A.1.1) family
Database Links

KEGG: sce:YKL217W

STRING: 4932.YKL217W

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