KAI2 Antibody

Code CSB-PA928241XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) KAI2 Polyclonal antibody
Uniprot No.
Target Names
KAI2
Alternative Names
KAI2 antibody; D14L antibody; HTL antibody; At4g37470 antibody; F6G17.120 antibody; Probable esterase KAI2 antibody; Protein DWARF-14-like antibody; Protein D14-like antibody; Protein HYPOSENSITIVE TO LIGHT antibody; Protein KARRIKIN INSENSITIVE 2 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana KAI2 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in seed germination and seedling development. Essential for plant responses to karrikins, a class of butenolide compounds, structurally similar to strigolactones, released from burning vegetation that stimulate seed germination and enhance seedling photomorphogenesis. KAI2 is not required for strigolactone-mediated responses, but MAX2 is necessary for responses to karrikins and strigolactones. Lacks detectable hydrolase activity against karrikin. Karrikin binding induces a conformational change.
Gene References into Functions
  1. KAI2 and MAX2 define a regulatory pathway that largely operates independently of HY5 to mediate seedling responses to abiotic signals such as smoke and light.[KAI2] PMID: 23142794
  2. KAI2-KAR1 creates a contiguous interface for binding signaling partners in a ligand-dependent manner. [KAI2] PMID: 23613584
  3. AtD14 and KAI2 define a class of proteins that permit the separate regulation of karrikin and strigolactone signalling by MAX2. [KAI2] PMID: 22357928
  4. HY5 directly binds to C/G-box and G-box in the HTL promoter and regulates the expression of HTL. [HTL] PMID: 20864454
Subcellular Location
Nucleus. Cytoplasm, cytosol. Note=Weak expression in the cytosol.
Protein Families
AB hydrolase superfamily
Tissue Specificity
In young seedlings, expressed in hypocotyls and roots. In adult plants, expressed in rosette leaves, stigma, sepals and silique peduncles and tips.
Database Links

KEGG: ath:AT4G37470

STRING: 3702.AT4G37470.1

UniGene: At.66602

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