KAR3 Antibody

Code CSB-PA322336XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) KAR3 Polyclonal antibody
Uniprot No.
Target Names
KAR3
Alternative Names
KAR3 antibody; YPR141C antibody; P9659.16 antibody; Kinesin-like protein KAR3 antibody; Nuclear fusion protein antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) KAR3 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Essential for yeast nuclear fusion during mating. KAR3 is a bifunctional protein having a kinesin-like motor domain joined to a distinct microtubule binding domain. It may mediate microtubule sliding during nuclear fusion and possibly mitosis. May interact with spindle microtubules to produce an inwardly directed force acting upon the poles. KAR3 function antagonizes CIP8 and KIP1 outward force action. KAR3 motor activity is directed toward the microtubule's minus end.
Gene References into Functions
  1. findings provide an example for a non-conventional translocation mechanism and can explain how Kar3 substitutes for key functions of Dynein in the yeast nucleus PMID: 25626168
  2. Kar3, with the help of its light chain, Cik1, is anchored during mating to the SPB component Spc72 that also serves as a nucleator and anchor for microtubules via their minus ends. PMID: 23388829
  3. the kar3 meiotic arrest may be mediated by the spindle checkpoint PMID: 14726698
  4. In Kar3, the extended alpha-helical domain NH2-terminal to the catalytic core provides the structural transitions in response to the ATPase cycle that are critical for motility; dimerization is not specifically required PMID: 15385545
  5. Kinetochores slide along the microtubule lateral surface, which is mainly and probably exclusively driven by Kar3. PMID: 17620411
  6. Kar3p contributes to spindle stability by cross-linking spindle microtubules. PMID: 18180364
  7. Gpa1 serves as a positional determinant for Kar3-bound microtubule plus ends during mating. PMID: 19386762

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Subcellular Location
Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body. Nucleus. Cytoplasm, cytoskeleton. Note=Cytoplasmic microtubules.
Protein Families
TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family, NCD subfamily
Database Links

KEGG: sce:YPR141C

STRING: 4932.YPR141C

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