FAE1 Antibody

Code CSB-PA655728XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) FAE1 Polyclonal antibody
Uniprot No.
Target Names
FAE1
Alternative Names
FAE1; KCS18; At4g34520; T4L20.100; 3-ketoacyl-CoA synthase 18; KCS-18; Protein FATTY ACID ELONGATION 1; Very long-chain fatty acid condensing enzyme 18; VLCFA condensing enzyme 18
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana FAE1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Contributes to fatty acids elongation and stockage in developing seeds. Active on both saturated and mono-unsaturated acyl-CoAs of 16 and 18 carbons. Required for the elongation of C18 to C20 and of C20 to C22 fatty acids. Mediates also the synthesis of VLCFAs from 20 to 26 carbons in length (e.g. C20:1, C20, C22:1, C22, C24:1, C24, C26). Has no activity with polyunsaturated C18:2 and C18:3 or with acyl-CoAs having 22 carbons or longer chain length.
Gene References into Functions
  1. We constructed a chimeric promoter that starts to express in the early stage of seed development, and high-level expression is retained until the later stage by connecting the promoters of the biotin carboxyl carrier protein 2 (BCCP2) gene encoding the BCCP2 subunit of acetyl-CoA carboxylase and the fatty acid elongase 1 (FAE1) gene from Arabidopsis PMID: 29874815
  2. KCS18 is a major control point for very long chain fatty acid synthesis in Arabidopsis. PMID: 23145136
  3. Nuclei of arrested trichome cells in FAE1 misexpression plants cell-autonomously accumulate high levels of DNA damage, including double-strand breaks characteristic of lipoapoptosis. PMID: 19376931
Subcellular Location
Microsome membrane; Multi-pass membrane protein.
Protein Families
Chalcone/stilbene synthases family
Tissue Specificity
Expressed specifically in seeds, especially in embryos. Expressed in siliques.
Database Links

KEGG: ath:AT4G34520

STRING: 3702.AT4G34520.1

UniGene: At.26989

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