KEA3 Antibody

Code CSB-PA864917XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) KEA3 Polyclonal antibody
Uniprot No.
Target Names
KEA3
Alternative Names
KEA3 antibody; At4g04850 antibody; T4B21.3 antibody; K(+) efflux antiporter 3 antibody; chloroplastic antibody; AtKEA3 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana KEA3 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Electroneutral K(+)/H(+) antiporter. Accelerates photosynthetic acclimation in fluctuating light environments.
Gene References into Functions
  1. Double mutations in the plastid K(+) exchange antiporter (KEA) transporters kea1kea2 and a single mutation that does not visibly affect chloroplast structure, kea3, impaired the rapid hyperosmotic-induced Ca(2+) responses. PMID: 27528686
  2. Findings suggest that fine regulation of K+ efflux antiporter KEA3 activity is necessary for optimizing photosynthesis. PMID: 27783435
  3. KEA3 activity is regulated by a mechanism involving its lumen-localized C-terminus, which lowers KEA3 activity in high light. PMID: 27335350
  4. KEA3 is critical for high photosynthetic efficiency under fluctuating light. PMID: 25451040
  5. role of inner envelope KEA1 and KEA2 and thylakoid KEA3 transporters in chloroplast osmoregulation, integrity, and ion and pH homeostasis PMID: 24794527

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Subcellular Location
Plastid, chloroplast membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Plastid, chloroplast thylakoid membrane; Multi-pass membrane protein.
Protein Families
Monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family, KEA (TC 2.A.37.1) subfamily
Tissue Specificity
Expressed in shoots and roots.
Database Links

KEGG: ath:AT4G04850

STRING: 3702.AT4G04850.2

UniGene: At.26531

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