KIP1 Antibody

Code CSB-PA326827XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) KIP1 Polyclonal antibody
Uniprot No.
Target Names
KIP1
Alternative Names
KIP1 antibody; CIN9 antibody; YBL063W antibody; YBL0504 antibody; YBL0521Kinesin-like protein KIP1 antibody; Chromosome instability protein 9 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) KIP1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Required for assembly of the mitotic spindle. Interacts with spindle microtubules to produce an outwardly directed force acting upon the poles. Following spindle assembly, CIN8 and KIP1 apparently act to oppose a force that draws separated poles back together. This force seems to be mediate by KAR3.
Gene References into Functions
  1. Clb2/Cdc28 drives spindle pole separation by direct phosphorylation of Kip1 and Cin8. PMID: 20463882
  2. spindle elongation in anaphase in Saccharomyces cerevisiae cells mutated for the kinesin-5 motor protein Kip1 PMID: 18628309
  3. Study finds in budding yeast that the widely conserved kinesin-5 sliding motor proteins, Cin8p and Kip1p, mediate chromosome congression by suppressing kMT plus-end assembly of longer kinetochore microtubules. PMID: 19041752
  4. Results show that the 2 micron plasmid of Saccharomyces cerevisiae uses the Kip1 motor, but not the functionally redundant Cin8 motor, for its precise nuclear localization and equal segregation. PMID: 19364922
Subcellular Location
Cytoplasm, cytoskeleton, spindle. Note=Spindle microtubules that lie between the poles.
Protein Families
TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family, BimC subfamily
Database Links

KEGG: sce:YBL063W

STRING: 4932.YBL063W

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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