KIP3 Antibody

Code CSB-PA345253XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) KIP3 Polyclonal antibody
Uniprot No.
Target Names
KIP3
Alternative Names
KIP3 antibody; YGL216WKinesin-like protein KIP3 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) KIP3 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Gene References into Functions
  1. The single kinesin-8 motor Kip3 alternately mediates both catastrophe and rescue of the bud microtubule (MT). Kip3 accumulates in a length-dependent manner along the lattice of MTs within the bud, yet induces catastrophe spatially near the bud tip. PMID: 25088560
  2. Kip3 directly suppresses spindle microtubule polymerization, limiting midzone length. PMID: 24616221
  3. the motion of yeast (Kip3) and human (Kif18A) kinesin-8s PMID: 23746518
  4. antiparallel microtubule-sliding activity of the budding yeast kinesin-8, Kip3 PMID: 23851487
  5. Studies indicate that the motor protein Kip3 (in the kinesin-8 family) is an ideal model system to reconstitute high-density traffic on microtubules. PMID: 22431622
  6. A model in which TORC1 and Clb5/CDK act coordinately to promote both spindle assembly via a pathway involving Kip3 and S phase progression. PMID: 20697716
  7. Kip3p is a highly processive, plus-end-directed motor, both in vitro and in vivo. PMID: 16906145
  8. Kip3p plays an essential role in positioning the mitotic spindle in budding yeast and potentially other processes controlled by kinesin-8 family members. PMID: 16906148
  9. characterization of frictional drag force of KIP3(kinesin-8 motor proteins) interacting with microtubule tracks; argue these frictional forces arise from breaking bonds between motor domains & the microtubule & they limit speed & efficiency of kinesin PMID: 19679813
  10. Study found that after binding at a random position on a microtubule and walking to the plus end, an individual Kip3p molecule pauses there until an incoming Kip3p molecule bumps it off. PMID: 19766569

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Subcellular Location
Cytoplasm, cytoskeleton.
Protein Families
TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family, Kinesin II subfamily
Database Links

KEGG: sce:YGL216W

STRING: 4932.YGL216W

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