Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) KIN14D Polyclonal antibody

Uniprot No.

F4JGP4

Target Names

KIN14D

Alternative Names

KIN14D antibody; ATK5 antibody; At4g05190 antibody; C17L7.110 antibody; C6L9.1Kinesin-like protein KIN-14D antibody; AtKIN14b antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana KIN14D protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Kinesin that supports microtubule movement in an ATP-dependent manner and that functions as a minus-end directed motor as well as a plus-end tracking protein. During mitosis, is involved in early spindle assembly. Participates in the capture of antiparallel interpolar microtubules and helps in generating force to coalign microtubules.

Gene References into Functions

ATK5 uses plus-end tracking to reach spindle midzones, where it then organizes microtubules via minus-end-directed motor activity [ATK5] PMID: 15659646
ATK5 participates in the search and capture of antiparallel interpolar microtubules, where it aids in generating force to coalign microtubules, thereby affecting spindle length, width, and integrity. PMID: 17220198
These results are among the first reports of any motor with a major role in anastral spindle structure in plants and demonstrate that the conservation of kinesin-5 motor function throughout eukaryotes extends to vascular plants. PMID: 17652157
Arabidopsis AtKIN14a and AtKIN14b genes encoding highly similar kinesins are two of the most closely related Arabidopsis paralogs. They together play important roles in controlling plant reproductive development. [AtKIN14b] PMID: 18088313

Subcellular Location

Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, phragmoplast. Note=In interphase, is enriched specifically at growing microtubule plus-ends. In dividing cells, accumulates in spindle midzones and phragmoplast leading edges.

Protein Families

TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family, KIN-14 subfamily

Tissue Specificity

Slightly expressed in anther lobes with pollen mother cells at anther stage 5. Strongly expressed at anther stage 6 in the tapetum and meiotic cells. Also detected in the gynoecium and the ovule.

Database Links

KEGG: ath:AT4G05190

STRING: 3702.AT4G05190.1

UniGene: At.33907

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KIN14D Antibody

Product Details

Customer Reviews and Q&A

Target Background