KIN7A Antibody

Code CSB-PA592584XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) KIN7A Polyclonal antibody
Uniprot No.
Target Names
KIN7A
Alternative Names
KIN7A antibody; HIK antibody; NACK1 antibody; At1g18370 antibody; F15H18.12Kinesin-like protein KIN-7A antibody; NPK1-activating kinesin-1 antibody; AtNACK1 antibody; Protein HINKEL antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana KIN7A protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Probable plus end-directed motor protein that functions in the NACK-PQR (ANP1-MKK6-MPK4) MAP kinase signaling pathway, which is essential for somatic cell cytokinesis, especially for the cell-plate formation and its expansion. Regulates the activity and the localization of ANP1, probably by association through the non-catalytic region of the kinase. Functionally redundant with NACK2 and essential to promote the progression of cytokinesis and for cellularization (formation of the cell plate) during microgametogenesis and megagametogenesis.
Gene References into Functions
  1. Using dissected Arabidopsis NACK1 molecules and AtNACK1-fused GFP, it was shown that the C-terminal tail of the stalk in addition to the motor domain is critical for its proper localization to the site of cell plate formation in the phragmoplast. PMID: 25502072
  2. Cytokinesis in A. thaliana is controlled by a pathway that includes HIK and a MAP kinase cascade that consists of ANPs and MKK6/ANQ. PMID: 20802223
  3. AtNACK1/HINKEL appears to be an essential component of a MAP kinase cascade that promotes cytokinesis of plant cells. PMID: 15569152
Subcellular Location
Cytoplasm, cytoskeleton, phragmoplast.
Protein Families
TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family, KIN-7 subfamily
Tissue Specificity
Expressed in roots, flowers, pollen mother cells and embryos.
Database Links

KEGG: ath:AT1G18370

STRING: 3702.AT1G18370.1

UniGene: At.26864

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