pykA Antibody

Code CSB-PA325114XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) pykA Polyclonal antibody
Uniprot No.
Target Names
pykA
Alternative Names
pykA antibody; b1854 antibody; JW1843Pyruvate kinase II antibody; EC 2.7.1.40 antibody; PK-2 antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) pykA protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Catalyzes the formation of pyruvate in the last step of glycolysis, it is irreversible under physiological conditions. The reaction is critical for the control of metabolic flux in the second part of glycolysis.
Gene References into Functions
  1. Knocking out pykA significantly increased the intracellular ATP level and thus significantly increased the levels of glucose consumption and biomass formation. PMID: 28363967
Protein Families
Pyruvate kinase family
Database Links
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